This process requires several step : after joining
TIMCELL,
we will contact you in the shortest time to complete the donor card which
will join the blood sample and determine in which Clinic will be the
delivery.
COLECTING:
will be done at the delivery by the obstetrician. After clamping and/or
sectioning of the cord, the blood obtained from placental vessels will be
colected in a sterile closed bag for avoiding bacterial contamination. Total
quantity that can be harvested is approximately 75\150 ml.
TRANSPORTING:
After the harvest, cord blood is
transported at room temperature 20/24 Celsius degrees in special recipients
to TIMCELL
laboratory where is processed.
TESTING:
Serological testing,
HLA-typization and determining of the total number of CD34+ cells (stem
cells) will be done in
TIMCELL
laboratory.

PROCESSING:
Processing of the cord blood consist in separation of the red cells and the
white cells (leucocytes). Then the red cells are discarded and the
mononuclear cells (MNC) from the upper “buffer” are recovered in a pellet
containing an important number of stem cells. The MNC pellet will be
cryopreserved.

FREEZING:
For the cryo-preservation the
cells are immerse in a solvent DMSO (10% dimetylsulfoxide) wich act as a
cryoprotectant.The purpose of the solvent is to protect cells against the
formation of ice crystals wich rupture cell membranes. For this goal is
important that the cells freez slowly. Final storage is in the vapor phase
of liquid nitrogen at temperature -196 degrees.
